Revvity, 6057708, PhenoPlate 384-well, black, optically clear flat-bottom, collagen-coated, lids, case of 160

Overview
These PhenoPlate 384-well microplates (formerly named CellCarrier Ultra microplates) for high content imaging applications are collagen-coated and are available in black in a 384-well format. The microplates benefit from a film bottom made of cyclic olefin, a plastic that has glass-like optical properties, providing superior image quality and also good cell adherence. They have an ultra-low plate bottom (0.2mm) for better well access when using water immersion and high NA objectives, and a new design, low profile polystyrene lid for reduced evaporation.
Extracellular matrix proteins such as collagen provide an attachment framework for the adhesion and growth of certain cell types in vivo, and can also be used for cell attachment to plate surfaces in vitro. Cellular fibronectin membrane proteins mediate the attachment of cells to collagen substrates. Collagen is the most abundant protein in mammals that is found throughout the body and is a major component of the extracellular matrix (ECM). The most frequently used types of collagen for coating are collagen I and IV. Collagen type I is suitable for endothelial and epithelial cells, muscle cells and hepatocytes. Collagen type IV is the major constituent of basement membranes and offers more physiologically relevant conditions to cells as well as improving the adherence of specific cell types i.e. PC-12 (rat adrenal pheochromocytoma cell line). Our catalog plates are coated with rat tail collagen Type I.
Packaging notes:

Cases of 40 are packaged as 2 sleeves of 20 plates, lids included
Cases of 160 are packaged as 8 sleeves of 20 plates, lids included

Well plate dimensions
Well format: 384-well

Description
Specification

Number of rows
16

Number of columns
24

Well volume
145 µL

Recommended working volume
25 µL - 120 µL

Height (mm)
14.35

Length (mm)
127.76

Width (mm)
85.48

Well diameter (mm)
3.26

Well depth (mm)
12.7

A1 to top offset (mm)
8.99

A1 to side offset (mm)
12.13

Well-to-well spacing (mm)
4.5 Color-Black/Clear Bottom
Plate Format-384 wells
Well Shape-Flat-bottom
Well Volume-145 µL
Working Volume-25 µL - 120 µL
Application-High Content Imaging Microscopy
Automation Compatible-Yes
Barcoding Compatibility-Yes
Brand-PhenoPlate
Detection Modality-Fluorescence
Format-Microplates
Lids Included?-Yes
Material-Cyclic Olefin
Packaging-8 sleeves of 20 plates
Quantity-160 plates
Sterility-Aseptic
Storage Conditions-4°C
Surface Treatment-Collagen coated