Revvity, 610VHHTLB, HTRF Anti-VHH Tb-Antibody, 5,000 Assay Points

Overview
Monoclonal anti-VHH antibody has been labeled with Tb crypate. Anti-VHH antibody binds to the native form of both alpaca and llama VHH and recombiant nanobodies. The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as nanobodies selection and titration.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here, anti-VHH-Tb binds to the nanobodies raised against protein A, while partner A* binds to a specific Ab labeled with an HTRF acceptor *partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with an acceptor for the detection.

 

 

Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a nanobody raised against protein A and a non-tagged partner A*. Dispense the two partners (10 µL): i.e. protein A+nanobody, add anti-VHH-Tb cryptate (total 5 µL), and anti-partner A labeled with acceptor (5 µL) Then incubate, and finally read. *partner A can also be biotinylated, tagged or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with an acceptor for the detection. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4
Product Group-Fluorescent Reagent
Shipping Conditions-Shipped Ambient
Target Class-Biomarkers
Technology-TR-FRET
Unit Size-20,000 assay points