Revvity, 62B42PEH, HTRF Human Amyloid-β 1-42 Detection Kit, 10,000 Assay Points

Overview
Amyloid β1-40 and β1-42 peptides are involved in the appearance of Alzheimer's Disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, β and ƴ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid β1-42 peptides in cell supernatant or in whole cells. The assay is a fast alternative to ELISA, thanks to the easy implementation of our Add and Read method.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Human amyloid beta 1-42 assay principle
The human amyloid beta 1-42 assay measures human amyloid 1-42 peptide in a cell or tissue supernatant or lysate. The assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the human beta amyloid 1-42 peptide. In presence of human beta 1-42 peptide in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing any changes caused by experimental variability under a no-wash assay format.

Human amyloid beta 1-42 assay protocol
The Human Amyloid beta 1-42 kit features a two-plate assay protocol, where cells are plated, stimulated, and optionally lysed in the same culture plate. Cell supernatants and/or lysates are then transferred to an assay plate for the detection of Human amyloid beta 1-42. This protocol enables the cells' viability and confluence to be monitored. The antibodies labeled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Unit Size-10,000 assay points