Revvity, 62BDBAR2PEB, HTRF β-arrestin 2 Recruitment Kit, 1,000 Assay Points

Overview
The B-arr2 recruitment cellular assay monitors the recruitment of Beta-arrestin 2 and AP2 through GPCR activation. The B-arr2 recruitment kit is used to characterize the effect of compounds on the Beta-arrestin signaling pathway, by monitoring the interaction between endogenous B-arrestin 2 and AP-2 as its partner.
It is known from the literature that pharmacological investigations on this interaction are relevant when investigating the Beta-arrestin signaling pathway in a GPCR context.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
B-arr2 recruitment kit assay principle
The B-arr2 recruitment assay monitors the recruitment of B-arrestin 2 mediated by GPCR activation in cells. Revvity's B-arr2 recruitment kit is highly sensitive, enabling the pharmacological characterization of compounds involving endogenous B-arrestin 2 and AP2 proteins. The kit uses two labeled antibodies, one coupled to a donor fluorophore, and the other to an acceptor. Both antibodies are highly specific for B-arr2 protein and AP2. In presence of B-arr2/AP2 interaction in cells, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the number of B-arrestin 2/AP2 complexes present in the sample.

B-arr2 recruitment assay protocol (option 1)
Detection of B-arr2 recruitment​​​​​​​ with HTRF reagents can be performed in a single plate used for culturing, stimulation, stabilization, and detection. Three washes are required after the stabilization step. This specific protocol enables detection with non-lysed cells and endogenous proteins after ON incubation at room temperature, while maintaining robust HTRF quality.

B-arr2 recruitment assay protocol (option 2)
Detection of B-arr2 recruitment​​​​​​​ with HTRF reagents can be performed in a single plate used for culturing, stimulation, stabilization and detection. Three washes are required after the stabilization step. This specific protocol enables detection with non-lysed cells and endogenous proteins, while maintaining robust HTRF quality after ON incubation at room temperature.
Option 2 of the B-arr2 recruitment assay protocol enables improvement of S/B by removing 80µL of detection reagents after ON incubation at room temperature. Application-Second Messenger Detection
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-200 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-GPCR
Technology-TR-FRET
Therapeutic Area-Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size-1,000 assay points