Revvity, 62BDNFPEG, HTRF BDNF Detection Kit, 500 Assay Points

Overview
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family, which also includes nerve growth factor (NGF) and neurotrophins 3 and 4 (NT-3 and NT-4).
BDNF is one of the most widely-studied neurotrophins in healthy or diseased brains. It is secreted by neurons and glial cells, and is strongly regulated by a wide variety of endogenous and exogenous stimuli (e.g. stress, physical activity, brain injury, diet). BDNF plays a crucial role in the control of neuronal and glial development, neuroprotection, and modulation of synaptic interactions, which are critical for cognition and memory.
Decreased levels of BDNF are associated with neurodegenerative diseases such as Parkinson’s Disease, Alzheimer’s Disease, Multiple Sclerosis, and Huntington’s Disease.
How it works
BDNF assay principle
BDNF is measured using a sandwich immunoassay involving two specific anti-BDNF antibodies, respectively labeled with an HTRF donor and acceptor dyes. The intensity of the signal is directly proportional to the concentration of BDNF present in the sample.

BDNF assay protocol
The assay protocol, using a 384-well small volume white plate or a Revvity low volume 96-well plate (20 µL final), is described on the right. 16 µL of cell supernatant or standard are dispensed directly into the detection plate for detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor can be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Neuroscience
Unit Size-500 assay points