Revvity, 62CRTPEH, HTRF Cortisol Detection Kit, 10,000 Assay Points

Overview
Cortisol, also known as hydrocortisone, is the major glucocorticoid produced and secreted by the adrenal cortex. It is involved in regulation of glucose metabolism, fat degradation, blood pressure levels, and stress inhibition. Cortisol also diffuses into cells, where its level is highly regulated by 11ß-hydroxysteroid dehydrogenase (type 1 or 2). The cortisol assay allows for rapid and accurate cortisol measurement, even in complex samples such as liver microsomes, whole cells, and animal serum. This assay can also be used to assess 11ß-HSD1 activity (native, recombinant, or microsomal).
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and cortisol coupled to d2 (acceptor). Cortisol produced by cells competes with d2-labelled cortisol for binding to monoclonal anti-cortisol Eu Cryptate.

Assay protocol
The cortisol assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Cortisol detection using HTRF reagents.
This protocol requires only a single, two-hour incubation period following cell stimulation. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped Ambient
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Metabolism/Diabetes
Unit Size-10,000 assay points