Revvity, 62CXCL12PEG, HTRF Human and Mouse CXCL12 Detection Kit, 500 assay points

Overview
CXCL12 (SDF-1) is a homeostatic chemokine constitutively expressed by bone marrow stromal cells, and is present in many other tissues (skin, thymus, lymph nodes, lung, liver). It plays an essential role in the homeostatic regulation of leukocyte traffic, hematopoiesis, organogenesis, cell differentiation, and tissue regeneration.
SDF-1 binds to and activates the CXCR4 receptor. CXCL12 and CXCR4 are overexpressed in various cancer types, and this aberrant expression strongly promotes proliferation, migration, and invasion through multiple signal pathways.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Principle of the HTRF CXCL12 assay

The human and mouse CXCL12 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+ cryptate (donor) and the other with d2 (acceptor). Both antibodies bind to CXCL12, and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of CXCL12 present in the sample.

Protocol of the HTRF CXCL12 assay

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF™ reagents (384-well low-volume white plate or Revvity low-volume 96-well plate, in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Dynamic Range-93.2 – 8000 pg/mL
Limit of Detection-1.8 pg/mL
Limit of Quantification-7.3 pg/mL
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-CXCL12
Target Class-Cytokines
Target Species-Human Mouse
Technology-TR-FRET
Therapeutic Area-Inflammation
Unit Size-500 assay points