Revvity, 62HCCL17PEH, HTRF Human CCL17 Detection Kit, 10,000 Assay Points

Overview
Human CCL17, also known as thymus and activation regulated chemokine (TARC), was initially isolated from phytohemagglutinin-stimulated peripheral blood mononuclear cells. CCL17 is constitutively expressed in the thymus and, under activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA+ CD4+ T cells plays a key role in allergic diseases such as atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. CCL17 and CCL22 secreted by dendritic cells (DCs) seem to mediate the recruitment of regulatory T cells to sites of inflammation in patients with chronic hepatitis.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Principle of the HTRF human CCL17 assay

The HTRF human CCL17 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+-cryptate (donor) and the other with d2 (acceptor). Both antibodies are directed against the CCL17 protein. They bind to soluble CCL17, and the donor-acceptor proximity leads to a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of soluble CCL17 present in the sample (cell supernatant).
 

 Protocol of the HTRF human CCL17 assay

The HTRF human CCL17 assay can be run in 96- or 384-well low volume white detection plates (20 µL final). As described here, samples (cell supernatants) or standards are dispensed directly into the assay plate for the detection of human CCL17 by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Dynamic Range-21 - 5500 pg/mL
Limit of Detection-20 pg/mL
Limit of Quantification-35.3 pg/mL
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-CCL17
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation
Unit Size-10,000 assay points