Revvity, 62HCCL22PEG, HTRF Human CCL22 Detection kit, 500 Assay Points

Overview
CCL22 (C-C motif chemokine 22), previously referred to as Macrophage-derived chemokine (MDC), is a chemokine secreted by dendritic cells (DC), macrophages, and activated B-lymphocytes. CCL22 binds to CCR4 present in many different cell types.
CCL22 is secreted by M2 macrophages, which contribute to Th2 responses such as phagocytosis, tissue repair, and wound healing.
Through its binding to CCR4, CCL22 has also been shown to contribute to the pathogenesis of atopic diseases, like asthma and atopic dermatitis (AD).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.  To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.  Cisbio also works with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CCL22 analysis. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Unit Size-500 assay points