Revvity, 62HIL02V2PEH, HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points

Overview
IL-2 plays a major role in modulating cell-mediated immunity through its effect on T cells. IL-2 promotes the differentiation of T cells into effector or memory T cells, thus helping the body fight off infections. IL-2 is also a key player in immuno-oncology where it serves as the main promoter of the differentiation of T cells into regulatory T cells (Tregs).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. For more information on performance metrics or to learn more about AlphaLISA, check out the Human IL-2 AlphaLISA kit and the Human IL-2 AlphaLISA biotin-free kit. When assaying, always follow the recommended protocols and avoid highly hemolyzed samples.
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Revvity also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL-2 analysis. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Unit Size-10,000 assay points