Revvity, 62HIL11PEG, HTRF Human IL-11 Detection Kit, 500 Assay Points

Overview
IL11, a member of the IL6 family of cytokines, regulates inflammatory responses and depending on the inflammatory microenvironnement, participates in cardiovascular diseases, cancers, or lung fibrosis.
IL11 is produced by a variety of stromal cells, including fibroblasts, epithelial cells, and osteoblasts, and has a variety of effects on hematopoiesis, on inhibition of adipocytogenesis, osteoclast differentiation, stimulating tissue fibrosis, minimizing tissue injury, and regulating the function of B cells.
After its secretion, IL11 binds to its receptor, which is composed of the IL11Ra chain associated with gp130 co-receptor, and triggers the jak/STAT signaling pathway.
HTRF human IL11 kit is a homogeneous add-and-read assay to measure IL11 secretion in cell supernatants. It offers a fast and efficient alternative to ELISA.
How it works
Assay Principle
Cell supernatant sample or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µL). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine release.
Revvity has also worked with Myassays.com to help you in your data analysis. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation NASH/Fibrosis
Unit Size-500 assay points