Revvity, 62HIL12P40PEG, HTRF Human IL-12 / IL-23 p40 Detection Kit, 500 Assay Points

Overview
p40 is a common component of the p40 family of cytokines which encompasses IL-12 (p40+p35 heterodimer), IL-23 (p40+p19 heterodimer), p40 (monomer), and p80 homodimer (p40+p40). Detection of the shared subunit p40 allows detection of all of these 4 members. These cytokines are instrumental in inflammation and immuno-regulation.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
Cell supernatant sample or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally.
 

Assay principle - p40 family
The assay is compatible with human samples only, and is highly specific for p40, cross-reacting with all p40-containing cytokines such as IL12, IL23, p40 monomer, and p40-p40 (p80) dimer.

Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also works with Myassays.com to help you in your data analysis. By clicking on this link, you'll be able to access a free online software to run your IL12/23 p40 analysis. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Dynamic Range-71 - 10,000 pg/mL
Limit of Detection-15 pg/mL
Limit of Quantification-71 pg/mL
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Unit Size-500 assay points