Revvity, 62HIL13PEG, HTRF Human IL-13 Detection Kit, 500 Assay Points

Overview
Interleukin 13 plays an essential role in allergic inflammation, airway disease, and asthma by regulating IgE synsis and inducing B-cell antibody class switching. IL13 can also induce differentiation of naive T-cells into Th2. Its effect on immune cells is similar to IL4 (close secondary structures). Outside immune cells, IL13 can induce matrix metalloproesases (MMPs) in fibroblasts as a mechanism to avoid asphixation during excessive allergic inflammation. Human IL13 is produced by Th2, CD4+, Tregs, NK, mast cells, basophils, and eosinophils, as well as epilial cells. After being secreted, it binds to IL13Ra1, which complexes with IL4Ra to form type II IL4R heterocomplex. This leads to activation of Jak1, Tyk2, and downstream signaling through STAT3/6.
HTRF human IL13 kit is a homogeneous, add and read assay to measure IL-13 secretion in cell supernatants. It offers a fast, efficient alternative to ELISA.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µL). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
 

Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing, and also 4PL 1/y² fitting,
Revvity also works to help you in your data analysis. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation
Unit Size-500 assay points