Revvity, 62HIL15PEG, HTRF Human IL-15 Detection Kit, 500 Assay Points

Overview
Human Interleukin 15 (IL-15) is a cytokine that is initially produced as a precursor that matures into a 114-amino acid glycoprotein. IL-15 is generated by multiple tissues, including the placenta, skeletal muscle, kidney, lung, heart, and monocytes/macrophages. IL-15 acts mainly on T and B cells and is a key cytokine in the development, proliferation, and survival of natural killer cells.
IL-15 binds with high affinity to the IL-15 receptor α and forms a complex with the α and γ chains of the IL-2 receptor for signal transduction. It can regulate inflammatory responses and activate NK cells during immune responses. IL-15 signaling has become a focus of interest for therapeutic interventions, especially in autoimmune diseases such as inflammatory bowel disease, systemic lupus erythematosus, inflammatory synovitis, psoriasis, diabetes mellitus, asthma bronchiale, rheumatoid arthritis, and T-cell leukemia.
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Unit Size-500 assay points