Revvity, 62HIL1B2PEG, HTRF Human IL-1β High Performance Detection Kit, 500 Assay Points

Overview
IL-1β, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of the IL1 family. IL1 beta is considered to be a major endogenous pyrogen and induces prostaglandin synthesis, T cell activation, B cell activation, and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-1β AlphaLISA kit's analytical performances for more information, or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
 

 

Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, 
Revvity also worked with Myassays.com to help you in your data analyses. You’ll be able to access a free online software to run your IL1 beta analysis. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human
Technology-TR-FRET
Unit Size-500 assay points