Revvity, 62HTMDPET, HTRF Histamine Dynamic Detection Kit, 96 Assay Points

Overview
Histamine (1H-imidazole 4-ethaneamine) is a biogenic amine known to play a key role in immediate hypersensitivity and inflammation reactions, as well as in several atopic diseases. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Bacterial decarboxylation of the amino acid histidine in fresh fish produces histamine, which can be used as a health hazard biomarker indicative of bacterial contamination. The histamine kit accurately measures histamine from PBMCs and cell-based formats.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and histamine coupled to d2 (acceptor). Histamine produced by cells competes with d2-labelled histamine for binding to monoclonal anti-histamine Eu Cryptate.
 

 

Assay protocol
The histamine assay features a streamlined protocol with only two incubation steps: - Sample with acylation buffer and acylation reagent - Histamine detection using HTRF reagents. This protocol requires a single, three-hour incubation period following the acylation step. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-6 µL
Shipping Conditions-Shipped Ambient
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Oncology & Inflammation
Unit Size-96 assay points