Revvity, 62IN1PEH, HTRF Insulin High Range Detection Kit, 10,000 Assay Points

Overview
Highly concentrated samples often need to be diluted prior to insulin being quantified in an assay. With no washing steps and a 4 log dynamic range, this HTRF High Range kit delivers on the promises of lower sample dilution and increased accuracy for a straightforward and reliable quantification of insulin.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
Insulin is measured using a sandwich immunoassay with two monoclonal antibodies, one labelled with Cryptate (donor) and the other with XL665 (acceptor). The intensity of the FRET signal obtained is proportional to the concentration of insulin in the sample.
 

 

Assay protocol
5 µL of cell supernatants are supplemented with 50 µL of acceptor-labelled antibodies and 25 µL of donor-labelled antibodies (or 75 µL of pre-mixed reagents for a single dispensing step). The mix is then incubated from 4h to overnight at RT and results are read on an HTRF compatible reader. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped Ambient
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Metabolism/Diabetes
Unit Size-10,000 assay points