Revvity, 62MMP1PEG, HTRF Human MMP1 Detection Kit, 500 Assay Points

Overview
Matrix metalloproteinase (MMP) 1, also known as interstitial collagenase, is an endopeptidase secreted as an inactive proenzyme that is then activated by proteolytic cleavage. MMP1 plays a significant role in tissue repair and remodeling by degrading structural components of the extracellular matrix (ECM), such as collagens, gelatin, and fibronectin. As such it is closely related to all matrix-dependent disorders, such as fibrotic disease, and some types of cancer that rely on cell adhesion, migration, and invasion.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Human MMP1 assay principle

The Human Matrix MetalloProteinase 1 (MMP1) assay measures MMP1 in cell supernatant. The assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. In presence of Human MMP1 in a sample, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the capsid present in the sample and therefore provides a means of assessing any changes caused by experimental variables, in a no-wash assay format.

Human MMP1 assay protocol

The Human MMP1 assay protocol, using a 384-well small volume white plate,  is described on the right. 16 µL of sample or standard are dispensed directly into the detection plate for detection by HTRF® reagents. The antibodies labeled with HTRF fluorophores are added before an overnight incubation. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Unit Size-500 assay points