Revvity, 63ADK056PEG, HTRF Human MANF Detection Kit, 500 Assay Points

Overview
Mesencephalic astrocyte-derived neurotrophic factor (MANF) (also recognized as ARMET or arginine-rich mutated in early stages of tumors) belongs to the fourth family of neurotrophic factors. MANF is an endoplasmic reticulum (ER) resident protein highly expressed in the developing cortex that plays an important role in protecting and repairing dopaminergic neurons in the rat 6-hydroxydopamine Parkinson disease model. MANF is also involved in protecting against various forms of endoplasmic reticulum stress. The human MANF kit enables the fast detection of MANF in cell supernatants and serum.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
Human MANF is measured using a sandwich immunoassay involving two specific human MANF antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of MANF present in the sample.

 

Assay protocol
The MANF assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 4-hour incubation period at RT. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Neuroscience
Unit Size-500 assay points