Revvity, 63ADK072PEG, HTRF acetyl K40 α-Tubulin Detection Kit, 500 Assay Points

Overview
Alpha-Tubulin is a member of the tubulin protein superfamily, which polymerizes with the beta tubulin to form microtubules, a major component of the eukaryotic cytoskeleton. Microtubules contribute to several cellular processes, such as structural support, intracellular transport, and DNA segregation. Alpha-Tubulin K40 acetylation has been shown to affect tubulin self-assembly and protect microtubules against stress-induced material fatigue. The Alpha-Tubulin acetyl-K40 kit is designed for the quantitative detection of K40 acetylated Alpha-Tubulin in cell lysates.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
Alpha-Tubulin acetyl-K40 is measured using a sandwich immunoassay involving two specific anti-alpha-Tubulin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of K40 acetylated alpha-Tubulin present in the sample.

 

Assay Protocol
The protocol for the alpha-Tubulin acetyl-K40 assay is described here. Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Alpha-Tubulin by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Molecular Modification-Acetylation
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Oncology & Inflammation
Unit Size-500 assay points