Revvity, 63ADK098PEG, HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points

Overview
Myosins are actin-based motor proteins that contribute to the generation of contractile force in eukaryotic cells. Each muscle myosin assembly contains a pair of myosin heavy chain (MYH) and two pairs of myosin light chain (MYL). MyHC-2 is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This assay enables the detection of Myosin Heavy Chain in cell lysates for research related to skeletal muscle diseases.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
Myosin Heavy Chain is measured using a sandwich immunoassay involving two specific anti-Myosin Heavy Chain antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the Myosin Heavy Chain present in the sample

 

Assay Protocol
The Myosin Heavy Chain assay protocol is described here. Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Myosin Heavy Chain. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Cardiovascular Rare Diseases
Unit Size-500 assay points