Revvity, 63ADK099PEG, HTRF Human TREM2 Detection Kit, 500 Assay Points

Overview
The kit is designed for the rapid detection of TREM-2 (also known as Triggering Receptor Expressed on Myeloid Cells 2) in cell supernatant and whole cells. The TREM family of receptors regulates the activity of various cell types of the immune system, including neutrophils, monocyte/macrophages, microglia, and dendritic cells. Soluble TREM-2 has been detected in human cerebrospinal fluid (CSF), where it was found to be elevated in patients with multiple sclerosis and other inflammatory neurological conditions in comparison to patients without inflammatory neurologic disorders.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
Human TREM2 is measured using a sandwich immunoassay involving two specific anti-human TREM2 antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the TREM2 present in the sample.

 

Assay protocol
The simple TREM2 assay protocol, using a 384-well small volume white plate (20 µL final), is described on the right. Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection of human TREM2. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Neuroscience Oncology & Inflammation
Unit Size-500 assay points