Revvity, 64AAV6PEG, HTRF AAV6 Detection Kit, 500 Assay Points

Overview
Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 6 (AAV6) is an efficient vector for gene delivery to skeletal muscle or lung tissue. The AAV6 Capsid kit is designed for the quantitative measurement of Adeno-associated virus serotype 6 (AAV6) particles in both cell lysates and cell supernatants.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
AAV6 Capsid assay principle
The Adeno-Associated Virus serotype 6 (AAV6) assay measures AAV6 Capsid in cell supernatant or cell lysate. The assay uses two anti-AAV6 antibodies: one coupled to HRP, that binds to anti-HRP d2 (the acceptor) in premix 1, and the other coupled to biotin, that binds to Streptavidin Eu-cryptate (the donor) in premix 2. In presence of AAV6 Capsid in a cell extract or supernatant, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the capsid present in the sample and provides a means of assessing any changes caused by experimental variability under a no-wash assay format.

 

 

AAV6 Capsid assay protocol
The AAV6 Capsid assay protocol using a 384-well small volume white plate is described on the right. 5 µL of sample or standard and 5 µL of diluent are dispensed directly into the plate for detection by HTRF® reagents. The Biotin anti-AAV6 antibody is pre-mixed with Streptavidin labeled with the donor, and HRP anti-AAV6 is pre-mixed with anti-HRP labeled with the acceptor. 5 µL of each premix are added. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Bioprocessing
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Viral Particles
Technology-TR-FRET
Unit Size-500 assay points