Revvity, 64AAV8PEH, HTRF AAV8 Detection Kit, 10,000 Assay Points

Overview
Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 8 (AAV8) when used as vector in gene therapy, has a liver cell transduction efficiency greater than those of other serotypes which has resulted in efforts to develop this virus as a gene therapy vector for hemophilia. AAV8 also exhibits a natural tropism for other tissues such as brain, muscle and retina. The AAV8 kit is designed to detect and quantify AAV8 particles in an easy-to-use, no-wash format. The simple and robust procedure benefits from increased throughput compared to ELISA.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
AAV8 capsid assay principle
The Adeno-Associated Virus serotype 8 (AAV8) assay measures AAV8 capsid in cell supernatant or cell lysate. The assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. In presence of AAV8 capsid in a cell extract or supernatant the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the capsid present in the sample and provides a means of assessing any changes caused by experimental variability under a no-wash assay format.

AAV8 capsid assay protocol
The AAV8 capsid assay protocol, using a 384-well small volume white plate,  is described on the right. 5 µL of sample or standard and 5 µL of diluent are dispensed directly into the detection plate for detection by HTRF® reagents. The antibodies labeled with HTRF fluorophores were added before an overnight incubation. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Bioprocessing
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Viral Particles
Technology-TR-FRET
Unit Size-10,000 assay points