Revvity, 64AAV9PEG, HTRF AAV9 Detection Kit, 500 Assay Points

Overview
Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 9 (AAV9) when used as a vector in gene therapy has been particularly developed for the treatment of neurological diseases mainly because of its ability to cross the blood-brain barrier to target the central nervous system (astrocytes and neurons). It also exhibits a tropism for other tissues such as liver, muscle or lung. The AAV9 kit is designed to detect and quantify AAV9 particles in an easy-to-use, no-wash format. The simple and robust procedure benefits from increased throughput compared to ELISA.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
AAV9 capsid assay principle
The Adeno-Associated Virus serotype 9 (AAV9) assay measures AAV9 capsid in cell supernatant or cell lysate. The assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. In presence of AAV9 capsid in a cell extract or supernatant the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the capsid present in the sample and provides a means of assessing any changes caused by experimental variability in a no-wash assay format.

AAV9 capsid assay protocol
The AAV9 capsid assay protocol, using a 384-well small volume white plate,  is described on the right. 5 µL of sample or standard and 5 µL of diluent are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labeled with HTRF fluorophores were added before an overnight incubation. The assay can be run in up to a 1536-well format by simply resizing each addition volume proportionally. Application-Bioprocessing
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Viral Particles
Technology-TR-FRET
Unit Size-500 assay points