Revvity, 64ARFLPEH, HTRF Androgen Receptor (FL mutant/AR-FL) Detection Kit, 10,000 Assay Points

Overview
The HTRF Human Full Length Androgen Receptor detection assay monitors Wild-Type Androgen Receptor (AR-WT) i.e. full length (AR-FL). It is used to detect the expression of endogenous or overexpressed AR-FL within various cells.
The Androgen Receptor is a nuclear receptor that is activated by androgen hormones, including testosterone (T) and Dihydrotestosterone (DHT).
Androgens bind to the ligand binding domain of the Androgen Receptor, resulting in receptor homodimerization and translocation to the nucleus. Downstream gene expression and signaling pathways are then activated, including the PI3K/AKT pathway. The gene expression induced by the Androgen Receptor promotes cell division, proliferation, differentiation, apoptosis, and angiogenesis. Dysregulation of the Androgen Receptor pathway can lead to oncogenic phenotypes, with Androgen Receptor activation or subexpression having been linked to conditions such as prostate cancer, breast cancer, glioblastoma, and other malignancies. Moreover, Androgen Receptor variants are expressed in various human cancer cell lines. The development of PROTAC compounds that target the degradation of the Androgen Receptor is a popular therapeutic strategy for targeting Androgen Receptor-positive cancers.
How it works
HTRF human full length androgen receptor assay principle

The HTRF Human Full Length Androgen Receptor assay quantifies the expression level of Androgen Receptor in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Human Androgen Receptor Full Length assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In presence of Androgen Receptor in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor, and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.

Human full length androgen receptor two-plate assay protocol
The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Human Full Length Androgen Receptor HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.

Human full length androgen receptor one-plate assay protocol
Detection of Human Full Length Androgen Receptor with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Phosphoproteins
Technology-TR-FRET
Unit Size-10,000 assay points