Revvity, 64ATA2PEH, HTRF Human and Mouse Ataxin 2 Detection Kit, 10,000 Assay Points

Overview
Ataxin 2 is an RNA-binding protein. In humans, Ataxin 2 causes neurodegeneration when carrying very long polyglutamine tract and drives disease progression in ALS. Current research in neurodegenerative and specifically ALS settings indicates there is a modulation of Ataxin 2 expression by TDP-43, which in turns sees its toxicity in cellular and animal models modified by Ataxin 2. The co-investigation of both proteins is a relevant topic for future therapeutic endeavours, with recent results indicating that lowered Ataxin 2 levels suppress TDP-43 aggregation.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Principle of the HTRF Human/Mouse Ataxin2 assay
The Human/Mouse Ataxin 2 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+ cryptate (donor) and the other with d2 (acceptor). Both antibodies bind to Ataxin 2 (WT & mutant forms), and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of Ataxin 2 present in the sample (cell lysate or tissue lysate).

 

Human/Mouse Ataxin2 assay assay protocol
The Human/Mouse Ataxin 2 assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples (cell/tissue lysates) or standards are dispensed directly into the assay plate for the detection of Ataxin 2 by HTRF® reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4 Lysis Buffer 5
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human Mouse
Technology-TR-FRET
Unit Size-10,000 assay points