Revvity, 64BAR1TPEB, HTRF Human and Mouse Total β-Arrestin 1 Detection Kit, 1,000 Assay Points

Overview
The Total B-arrestin 1 cellular assay monitors total B-arrestin 1, and is used to detect the expression of endogenous or overexpressed B-arrestin 1 in various cells. The B-arrestin 1 are of the n/beta-arrestin protein family, and wn to be implicated in the -mediated desensitization of G-protein-coupled receptors. They also cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. As B-arrestin 1 is expressed in various tissues, it is believed that B-arrestin 1 may play a major role in many cancers or other human diseases (diabetes) by ing receptor-mediated ns.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Total B-arrestin 1 assay principle
The Total B-arrestin 1 assay quantifies the expression level of B-arrestin 1 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total B-arrestin 1 assay uses two labeled antibodies: One coupled to a donor fluorophore, the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In the presence of B-arrestin 1 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.

 

Total B-arrestin 1 two-plate assay protocol
The 2 plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a 384-well low volume detection plate before the addition of the Total B-arrestin 1 HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.

 

Total B-arrestin 1 one-plate assay protocol
Detection of total B-arrestin 1 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-GPCR
Target Species-Human Mouse
Technology-TR-FRET
Therapeutic Area-Cardiovascular Inflammation Metabolism/Diabetes NASH/Fibrosis
Unit Size-1,000 assay points