Revvity, 64BDSTGHPEG, HTRF Human H232 STING Binding Kit, 500 Assay Points

Overview
A fast and easy way to identify new binders to human H232 STING.
Stimulator of interferon genes (STING), also known as transmembrane protein 173 (TMEM173), is a protein playing a major role in innate immunity.
The H232 variant is found in 13.7% of the world population. Upon intracellular cytosolic DNA release from pathogens such as viruses and bacteria, 2’-3’cGAMP binds to STING protein and triggers the secretion of type 1 interferon. However, the H232 variant is known to poorly bind 2'-3'cGAMP and other natural dinucleotides. The quest for molecules that are effective against all genetic variants has become crucial to modulate immunity in autoinflammatory diseases.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assy principle
The HTRF H232 STING binding assay is a competitive assay format which uses d2-labeled H232 STING ligand, a 6His tagged human H232 STING protein, and an anti 6His Cryptate-labeled antibody. Your compound competes with the H232 STING ligand-d2 and thereby prevents FRET from occurring.

Assay protocol
The Human H232 STING binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The human His-tagged H232 STING protein is then added, followed by the dispensing of the HTRF reagents: the anti 6His antibody labeled with Terbium cryptate and  the H232 STING ligand labeled with d2. The reagents labeled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein-Protein Interaction
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Binding Assay
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Infectious Diseases Oncology & Inflammation
Unit Size-500 assay points