Revvity, 64BDSTGQPEH, HTRF Human AQ STING Binding Kit, 10,000 Assay Points

Overview
A fast and easy way to identify new binders to human AQ STING.
Stimulator of interferon genes (STING), also known as transmembrane protein 173 (TMEM173), is a protein playing a major role in innate immunity.
The AQ and HAQ variants are found in respectively 5.2% and 20.4% of the world population. Both variants display the same binding pocket, since the R71H mutation is part of the transmembrane domain, and not expressed in the recombinant protein.
Upon intracellular cytosolic DNA release from pathogens such as viruses and bacteria, 2’-3’cGAMP binds to STING protein and triggers the secretion of type 1 interferon. The AQ variant shows a strong affinity to natural 2'-3'cGAMP and other known compounds.
Identifying new STING ligands is therefore a way to control immunity and fight autoinflammatory diseases.
How it works
Assay principle
The HTRF AQ STING binding assay is a competitive assay format which uses d2-labeled AQ STING ligand, a 6His tagged human AQ STING protein, and an anti 6His Cryptate-labeled antibody. Your compound competes with the AQ STING ligand-d2 and thereby prevents FRET from occurring.

 

Assay protocol
The Human AQ STING binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The human His-tagged AQ STING protein is then added, followed by the dispensing of the HTRF reagents: anti 6His antibody labeled with Terbium cryptate and AQ STING ligand labeled with d2. The reagents labeled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein-Protein Interaction
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-5 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Binding Assay
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Infectious Diseases Oncology & Inflammation
Unit Size-10,000 assay points