Revvity, 64BDVHLRED, HTRF VHL Red Ligand

Overview
HTRF VHL-Red Ligand is primarily intended to perform affinity binding curves to VHL protein using HTRF® technology.
VHL, also known as von Hippel-Lindau, is involved in many biological processes and is closely associated with the regulation of angiogenesis, cell growth, or cell survival. VHL is one of the most popular E3 ligases recruited by bifunctional Proteolysis-targeting chimeras (PROTACs) to induce ubiquitination and subsequent proteasomal degradation of a targeted protein. VHL interacts with several proteins to form the functional von Hippel-Lindau ubiquitination complex, in which VHL seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and targets various proteins to proteolysis, such as the hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions.
Identifying new PROTAC VHL ligands can improve selective proteasomal-dependent degradation of proteins of interest involved in the onset of diseases such as cancers.
The HTRF VHL-Red Ligand is primarily intended to perform cooperativity binding studies. It enables calculation of the affinity of the VHL-Red Ligand for VHL protein in the presence of the targeted protein of interest. The determination of the Bmax and the Kd of HTRF VHL-Red Ligand for VHL protein, in absence and presence of the targeted protein, enables the accurate preliminary analysis required for studying cooperative effects assessed with the HTRF Human VHL Binding Kit.
How it works
HTRF VHL ligand binding assay principle
HTRF VHL-Red Ligand binding is detected in a direct binding assay format, using an anti 6His-Europium Cryptate antibody which binds to a 6His-tagged Human VHL protein complex. When the dyes are in close proximity, the excitation of the donor with a light source (laser or flash lamp) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor, which in turn fluoresces at a specific wavelength (665 nm) (Image A). The specific binding signal is calculated by subtracting the non-specific binding signal from the total signal, enabling Kd determination for the HTRF VHL Red Ligand (Image B).

 

HTRF VHL-Red Ligand binding assay protocol
VHL saturation binding experiments of HTRF VHL-Red Ligand can be run in 384-well plates by dispensing 5 µL of Diluent 9 buffer (for Total binding) or VHL Binding standard (for non specific binding). Then 5 µL of PROTAC Binding Buffer 1 (for reference) or the targeted protein diluted in PROTAC Binding Buffer 1 (for cooperativity studies) are added, followed by 5 µL of 6His-tagged VHL protein complex. Finally, 5 µL of a pre-mixed solution of HTRF VHL-Red Ligand and Anti-6His Europium cryptate antibody are added.
The HTRF Ratio is measured after 1 hour of incubation at room temperature. Automation Compatible-Yes
Brand-HTRF
Detection Modality-HTRF
Product Group-Fluorescent Reagent
Shipping Conditions-Shipped in Dry Ice
Technology-TR-FRET
Therapeutic Area-Inflammation Neuroscience Oncology & Inflammation
Unit Size-1 unit