Revvity, 64CHOPEG, HTRF CHO HCP Detection Kit, 500 Assay Points

Overview
The CHO Host Cell Protein (HCP) kit measures contaminants originating from the CHO cells used to manufacture therapeutic antibodies. The kit can be used for the quantification of CHO HCP proteins in routine bioprocess operations, from the completely raw harvest material to the final product. The simple and robust procedure benefits from increased throughput compared to ELISA.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The CHO Host Cell Protein (HCP) assay is a sandwich immunoassay. The assay uses a specific and qualified anti-CHO HCP polyclonal antibody pool, labelled with Eu3+-Cryptate and t d2. The specific signal is proportional to the concentration of CHO HCP present in the sample. This homogeneous assay requires only a single reagent addition (by premixing the detection reagents), and an overnight incubation, for the quantitative detection of CHO HCP.

 

Assay protocol
The CHO HCP assay protocol, using a 384-well small volume white plate, is described on the right. 16 µL of sample or standard are dispensed directly into the detection plate for detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor can be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. This protocol requires only 1 overnight incubation period. Application-Protein Quantification
Automation Compatible-Yes
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Technology-TR-FRET
Therapeutic Area-Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Rare Diseases
Unit Size-500 assay points