Revvity, 64DAGGRPEG, HTRF Protein Disaggregation Kit, 500 Assay Points

Overview
This kit is intended for the simple and rapid disaggregation of several aggregated proteins, such as Amyloid beta 1-42, TDP-43, or alpha-synuclein. Aggregated proteins are produced by cells, and can be disaggregated after lysis of the cell membrane or directly after secretion in cell culture media. The level of disaggregated protein in cell lysate or in cell culture media can be detected using the appropriate immunoassay corresponding to the targeted protein.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Protein Disaggregation Kit Principle
The disaggregation principle is based on two successive reagent dispensing steps, without the need for centrifugation. Disaggregation buffer A is added to the sample and left for 15 min at room temperature, then Disaggregation buffer B is added. The same sample is also treated by Control detection buffer C to generate an appropriate control. After treatment, samples are ready to use in immunoassays. Signals from samples containing protein aggregates (treated with Control buffer C) can be compared to the same samples treated with Disaggregation reagents A and B. The ratio obtained between disaggregated/ aggregated signals on the same samples is proportional to level of protein aggregation.

 

Protein Disaggregation Kit Protocol

 
 

Sample

Disaggregation buffer A

Disaggregation buffer B

Control detection buffer C

Control detection buffer C

Sample

Disaggregation

60 µL

10 µL

10 µL

 

 

Control

60 µL

-

 

10 µL

10 µL Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Technology-TR-FRET
Unit Size-500 assay points