Revvity, 64DDR1TPEH, HTRF Human Total DDR1 Detection Kit, 10,000 Assay Points

Overview
DDR (Discoidin Domain Receptor family member 1) is a transmembrane tyrosine kinase receptor found in epithelial and mesenchymal cells. it binds and is activated by collagen and is a key player in cell-matrix interaction, with roles in adhesion and migration. These functions make it a potentail target of interest in matrix-related disorders such as fibrosis, but also some types of cancer wher cell migration and invasion is critical.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Total DDR1 assay principle

This Total DDR1 assay quantifies the expression level of DDR1 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total DDR1 assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In the presence of DDR1 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein's expression under a no-wash assay format.

Total DDR1 two-plate assay protocol

The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of HTRF Total DDR1 detection reagents. This protocol enables the cells' viability and confluence to be monitored.

Total DDR1 one-plate assay protocol

Detection of Total DDR1 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Application-Cell Signaling
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 4
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Phosphoproteins
Technology-TR-FRET
Unit Size-10,000 assay points