Revvity, 64FLTREM2PEH, HTRF Human TREM2 Full-Length Detection Kit, 10,000 assay points

Overview
The kit is designed for the rapid detection of the full-length TREM2 receptor by using two antibodies that target distinct regions, the extracellular domain and the intracellular region, allowing detection before receptor shedding by ADAM10 and ADAM17 proteases. TREM2 (Triggering Receptor Expressed on Myeloid Cells 2) belongs to the TREM family of receptors, which regulate the activity of various immune cell types, including neutrophils, monocytes/macrophages, microglia, and dendritic cells.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in readout time or size of assays
How it works
HTRF TREM2 full-length assay principle
The TREM2 full-length assay measures full-length TREM2 levels in cells. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. It uses two antibodies: one labeled with a donor fluorophore and the other with an acceptor. Both antibodies are highly specific for distinct epitopes on the protein.
In the presence of full-length TREM2, an immune complex forms involving both labeled antibodies, bringing the donor fluorophore into close proximity to the acceptor and generating a FRET signal. The signal intensity is directly proportional to the concentration of full-length TREM2 in the sample.

 

HTRF TREM2 full-length two-plate assay protocol
The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of TREM2 Full-Length HTRF detection reagents. This protocol allows the cells' viability and confluence to be monitored.

HTRF TREM2 full-length one-plate assay protocol
Detection of TREM2 full-length with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol allows miniaturization while maintaining robust HTRF quality. Application-Cell Signaling
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 3 Lysis Buffer 4
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-TREM2
Target Class-Phosphoproteins
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Neuroinflammation Neuroscience
Unit Size-10,000 assay points