Revvity, 64HFNEDAPEG, HTRF Human Fibronectin EDA Detection Kit, 500 Assay Points

Overview
Fibronectin is a large glycoprotein of the extracellular matrix whose functions are connected to cell adhesion, growth, migration, and differentiation. Fibronectin level regulation is strongly related to inflammation and fibrotic disorders, as well as to the matrix re-organization that occurs in the environment of some cancers. Tumor containment, migration, and expansion are critically dependent on a tumor's surrounding matrix and on key adhesive and/or fibrous proteins such as fibroncetin.
The EDA domain of fibronectin is an alternatively spliced version of fibronectin that is almost entirely absent from healthy tissues, and mostly expressed in active fibroblasts at injury or inflammation sites. Because of this special feature, EDA-fibronectin is a marker that is specific to fibroblast-induced fibrotic disorders, as well as being a readout of pro-fibrotic activity in a tumor matrix environment.
How it works
Human Fibronectin EDA assay principle
Human Fibronectin EDA is measured using a sandwich immunoassay involving specific anti-Fibronectin EDA antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of Fibronectin EDA present in the sample.

 

Human Fibronectin EDA assay protocol
The Human Fibronectin EDA assay protocol is shown here using a 384-well small volume white plate (20 µL final). Cell supernatant, whole cell lysate, sample, or standard is dispensed directly into the assay plate for the detection of Fibronectin EDA by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally. Application-Protein Quantification
Automation Compatible-Yes
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation NASH/Fibrosis Oncology & Inflammation
Unit Size-500 assay points