Revvity, 64MERTKTPEH, HTRF Human Total MerTK Detection Kit, 10,000 Assay Points

Overview
MerTK (Myeloid-Epithelial-Reproductive Tyrosine Kinase) is a transmembrane tyrosine kinase receptor predominantly expressed in dendritic cells, macrophages, epithelial cells, and cells of the reproductive tract. When stimulated by ligands such as Protein S or Gas6, activated MerTK confers a survival advantage to cancer cells by promoting their survival, enhancing migration, and inhibiting apoptosis. The overexpression of MerTK is implicated in a wide range of cancers, making it an intriguing target for cancer therapies research.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Total MerTK assay principle
The Total MerTK assay quantifies the expression level of MerTK in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total MerTK assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In the presence of MerTK in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample and provides a means of assessing the protein's expression under a no-wash assay format.

Total MerTK two-plate assay protocol
The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of HTRF Total MerTK detection reagents. This protocol allows for the cells' viability and confluence to be monitored.

Total MerTK one-plate assay protocol
Detection of Total MerTK with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol facilitates miniaturization while maintaining robust HTRF quality. Application-Cell Signaling
Brand-HTRF
Buffer/Solvent-Lysis Buffer 4
Detection Modality-HTRF
Host Species-Human
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-MerTK
Target Class-Phosphoproteins
Technology-TR-FRET
Therapeutic Area-Inflammation Oncology
Unit Size-10,000 assay points