Revvity, 64MSH3TPEG, HTRF Total MSH3 Detection Kit, 500 Assay Points

Overview
MSH3, also known as MutS Homolog 3, is a protein that plays a key role in DNA mismatch repair (MMR), a mechanism that ensures the fidelity of DNA replication and maintains genomic stability over time. The MSH3 protein fulfills its function as part of a MutS heterodimeric complex which includes another MutS homolog, typically MSH2.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
HTRF Human Total MSH3 assay principle
The MSH3 assay measures MSH3 in cells. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The assay uses 2 antibodies, one labeled with a donor fluorophore and the other with an acceptor. MSH3 binding by the antibodies enables an immune-complex formation which brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. Its intensity is directly proportional to the concentration of MSH3 protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.

HTRF Human Total MSH3 one-plate assay protocol
Detection of Total MSH3 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.

HTRF Human Total MSH3 two-plate assay protocol
The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a 384-well low volume detection plate before the addition of Total MSH3 HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored. Application-Cell Signaling
Brand-HTRF
Detection Modality-HTRF
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Phosphoproteins
Technology-TR-FRET
Unit Size-500 assay points