Revvity, 64SALL4TPEG, HTRF Human Total SALL4 Detection Kit, 500 Assay Points

Overview
SALL4 is a transcription factor from the spalt-like (SALL) gene family, known for its zinc finger domains and crucial role in maintaining the pluripotency and self-renewal of embryonic stem cells. Although SALL4 expression decreases significantly in most adult tissues, it is aberrantly reactivated in various cancers, promoting cell proliferation and stem cell-like characteristics in malignant cells. This re-expression links SALL4 to tumorigenesis and poor prognosis, making it a notable biomarker and potential therapeutic target in oncology.
Immunomodulatory imide drugs (IMIDs), including lenalidomide and thalidomide derivatives, have shown promise in targeting SALL4, especially the SALL4A isoform. These drugs have demonstrated anti-tumor activity but pose a significant risk due to their teratogenic effects, causing severe birth defects. Despite this limitation, SALL4 remains an attractive target in aggressive cancers where its expression is aberrant, especially when other treatments have failed. Ongoing research aims to develop more selective and less toxic inhibitors, balancing the therapeutic benefits of targeting SALL4 with the risks of teratogenicity, thus offering new potential in cancer treatment.
How it works
Total SALL4 assay principle
The Total SALL4 assay quantifies the expression level of SALL4 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total SALL4 assay uses two labeled antibodies, one coupled to a donor fluorophore and the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In the presence of SALL4 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample and provides a means of assessing the protein's expression under a no-wash assay format.

 

Total SALL4 two-plate assay protocol
The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of HTRF Total SALL4 detection reagents. This protocol allows the cells' viability and confluence to be monitored.

 

Total SALL4 one-plate assay protocol
Detection of Total SALL4 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol allows for miniaturization while maintaining robust HTRF quality. Application-Protein Quantification
Brand-HTRF
Buffer/Solvent-Lysis Buffer 4
Detection Modality-HTRF
Host Species-Human
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-SALL4
Target Class-Phosphoproteins
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation Oncology
Unit Size-500 assay points