Revvity, 64SLPY5TDA, HTRF Human Phospho-SLP76 (Tyr145) Detection Kit, Control Lysate

Overview
This HTRF cell-based assay enables rapid, quantitative detection of SLP-76 phosphorylated on Tyrosine 145. Phosphorylation of SLP-76 by ZAP-70 kinase creates a scaffold on which key signaling complexes are built in order to trigger T-lymphocyte activation. SLP-76 (Tyr145) is considered as a direct readout of ZAP-70 activation pathway in T-immune cells.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays Application-Cell Signaling
Automation Compatible-Yes
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 2 Lysis Buffer 3 Lysis Buffer 4
Molecular Modification-Phosphorylation
Product Group-Control Lysate
Shipping Conditions-Shipped in Dry Ice
Target Class-Phosphoproteins
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Oncology & Inflammation
Unit Size-1 vial