Revvity, 64STREM2PEG, HTRF Human and Mouse Soluble TREM2 Detection Kit, 500 assay points

Overview
This kit enables rapid and reliable detection of TREM-2 (Triggering Receptor Expressed on Myeloid Cells 2) in cell supernatants. TREM receptors play a key role in regulating immune cell activity, including neutrophils, monocytes/macrophages, microglia, and dendritic cells. Soluble TREM-2 has been identified in human cerebrospinal fluid (CSF) and shown to be elevated in patients with multiple sclerosis and other inflammatory neurological disorders compared to individuals without such conditions.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in readout time or size of assays
How it works
Principle of the HTRF human and mouse soluble TREM2 assay
The HTRF Soluble TREM2 assay is based on a TR-FRET sandwich immunoassay involving two specific antibodies, one labelled with Eu3+ cryptate (donor) and the other with d2 (acceptor). Both antibodies bind to soluble TREM2, and the donor-acceptor proximity enables a fluorescent TR-FRET signal. The intensity of the signal is directly proportional to the concentration of soluble TREM2 present in the sample.

 

Protocol of the HTRF Human and Mouse Soluble TREM2 assay
The HTRF Soluble TREM2 assay can be run in a 96- or 384-well low volume white detection plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate for the detection of soluble TREM2 by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Dynamic Range-81 - 12,000 pg/mL
Limit of Detection-5 pg/mL
Limit of Quantification-22 pg/mL
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-TREM2
Target Class-Biomarkers
Target Species-Human Mouse
Technology-TR-FRET
Therapeutic Area-Neuroinflammation Neuroscience
Unit Size-500 assay points