Revvity, 64WRNTPEH, HTRF Human Total WRN Detection Kit, 10,000 assay points

Overview
WRN is a protein of the helicase family involved in genome stability-related process including DNA repair, replication, and maintenance via telomere stability. Abnormal WRN functions characterize Werner Syndrome, which is a rare genetic disorder where genome stability is compromised, leading to the accumulation of senescence factors and premature aging in patients. As a disorder related to genome stability and degenerescence, it is also strongly related to increased cancer risks.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
WRN assay principle

The WRN assay measures WRN levels in cells. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The assay uses 2 antibodies, one labeled with a donor fluorophore and the other with an acceptor. In the presence of WRN, the labeled antibodies form an immune complex, bringing the donor fluorophore into close proximity to the acceptor and generating a FRET signal. The signal intensity is directly proportional to the concentration of total protein present in the sample.

WRN two-plate assay protocol

The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of WRN HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored

WRN one-plate assay protocol

Detection of WRN with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol allows miniaturization while maintaining robust HTRF quality. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1
Molecular Modification-Total
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target-WRN
Target Class-Biomarkers
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Inflammation Oncology Rare Diseases
Unit Size-10,000 assay points