Revvity, 65EU2ABA, HTRF Europium NH2 Labeling Reagent, 100 µg

Overview
Europium cryptate-NH2, is a do-it-yourself solution for labeling peptides, oligonucleotides or proteins with Europium cryptate.This reagent is ideal for experienced users familiar with labeling and chromatography. Used in combination with bi-functional reagents such as carbodiimides and SMCC/SPDP. Maleimide group generated by SMCC activation of the Europium cryptate-NH2 reacts with the thiol generated by SPDP/DTT treatment on the biomolecules to label.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay protocol
As shown here, preactivation of both the Europium cryptate and the biomolecule may be required. Recovery of labeled material can be achieved using conventional chromatographic methods, such as gel filtration or HPLC. The purification devices required for recovering labeled material are not delivered with these reagents. Application-Labeling
Brand-HTRF
Detection Modality-HTRF
Product Group-Fluorescent Reagent
Shipping Conditions-Shipped in Dry Ice
Technology-TR-FRET
Unit Size-100 µg