Revvity, 6FHIGPEG, HTRF Human IgG Detection Kit, 500 Assay Points

Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG kit enables a rapid quantification of human IgG in cell supernatant and the selection of the best producer clones. The assay is compatible with natural IgG as well as recombinant antibodies.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
This kit is a sandwich immunoassay enabling highly sensitive quantification of human IgG, even when using a small sample volume. The assay uses two polyclonal antibodies, one (Fc specific) labelled with Cryptate and the second (FAb specific) with d2. The signal intensity is proportional to the number of antibody-antibody complexes formed and therefore to the human IgG concentration.

Assay Protocol
The human IgG assay features a streamlined protocol with only 1 incubation step after sample and human IgG detection reagents dispensing. This protocol requires a single two-hour incubation period at RT. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biologics
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Rare Diseases
Unit Size-500 assay points