Revvity, 6FHSAPEG, HTRF Human HSA Detection Kit, 500 Assay Points

Overview
Human Serum Albumin (HSA) is a protein produced in the liver that represents the most abundant component in human blood plasma. Serum Albumin's main function is the regulation of the colloidal osmotic pressure of blood. The kit is intended for the specific and fast quantification of HSA in cell supernatants.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The HSA assay is a sandwich immunoassay. The assay uses two specific anti-human serum albumin antibodies, one labelled with Eu3+-Cryptate and the other with d2. The specific signal is proportional to the concentration of HSA present in the sample. This homogeneous assay requires only a single reagent addition (by premixing detecting reagents) and an ON incubation for the quantitative detection of CHO HCP.

Assay Protocol
The HSA assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires only a single ON incubation period at RT. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biologics
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Metabolism/Diabetes
Unit Size-500 assay points