Revvity, 6FMIGPEH, HTRF Mouse IgG-Fc Detection Kit, 10,000 Assay Points

Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Mouse Fc kit enables a rapid quantification of mouse IgG, and any proteins containing a mouse Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
HTRF assays offer many advantages over other technologies:

Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The mouse Fc kit is a competitive immunoassay for the measurement of mIgGs or any mFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to mFc and XL665 coupled mIgGs. mFc contained in the sample competes with XL665-labelled mouse IgG for binding to anti-mouse Fc Eu Cryptate.

Assay Protocol
The mouse Fc assay features a streamlined protocol with only 1 incubation step after sample and Mouse Fc detection reagents dispensing. This protocol requires a single two-hour incubation period. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-10 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biologics
Target Species-Mouse
Technology-TR-FRET
Therapeutic Area-Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Rare Diseases
Unit Size-10,000 assay points