Revvity, 72IP1PEA, ELISA IP-One Detection Kit, 96 Assay Points

Overview
GPCR Gq stimulation is known to induce phospholipase C (PLC) activation and trigger Inositol phosphate (IP) cascade.
IP1, a downstream metabolite of IP3, accumulates in cells following Gq receptor activation and is stable in presence of LiCl, making it an ideal GPCR (Gq) functional assay.
Revvity has developed a monoclonal antibody-based IP-One ELISA to enable simplified and highly accurate measurement of IP1.
How it works
Assay principle
IP-One ELISA is a competitive immunoassay which uses IP1-HRP and an anti-IP1 monoclonal antibody. The kit comes with 96-well microplates pre-coated with an anti-mouse antibody. Cells are stimulated in the presence of LiCl, causing the accumulation of IP1 upon receptor activation. The protocol consists of two steps following cell stimulation: addition of ELISA components and addition of TMB, the HRP substrate. The HRP reaction is stopped and the optical density (OD) read at 450nm.

Assay protocol Application-Second Messenger Detection
Detection Modality-ELISA
Dynamic Range-10 - 3,000 nM
Limit of Detection-10 nM
Product Group-Kit
Sample Volume-50 µL
Shipping Conditions-Shipped Ambient
Target Class-GPCR
Technology-ELISA
Therapeutic Area-Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size-96 assay points