Revvity, 81BOOSTR1, pHSense Boost Reagent, 0.5 mL

Overview
pHSense Eu reagents are europium-labeled, pH-sensitive probes designed for the real-time, no-wash detection of antibody internalization, as well as receptor- and membrane protein-mediated internalization in live-cell, plate-based assays.
pHSense Boost is an innovative reagent developed specifically to enhance pHSense Eu internalization assays by addressing the limitations of narrow assay windows. It effectively reduces background signals and amplifies fold-change measurements, improving assay dynamic range.
A key advantage of pHSense Boost is its ability to enhance assay performance without affecting the pharmacological profiles of tested compounds, ensuring the integrity and reliability of screening and characterization data.
The efficacy of pHSense Boost is influenced by cell type, cell density, and incubation time. As such, its benefits are not universal and require empirical validation for each specific assay condition.
How it works
pHSense Boost reagent: internalization assay principle
pHSense Eu reagents are europium-labeled, pH-sensitive probes that enable the real-time, no-wash detection of antibody internalization, as well as receptor- and membrane protein-mediated internalization in live-cell, plate-based assays.
pHSense Boost is a novel reagent specifically designed to optimize pHSense Eu internalization assays by addressing the limitations of narrow assay windows. It reduces background signals and enhances fold-change measurements, improving assay performance.
The efficacy of pHSense Boost depends on cell type, cell density, and incubation time. Its benefits are not universal, and must be empirically validated for each specific assay condition. Application-Internalization
Brand-pHSense
Detection Modality-pH sensitive dye
Product Group-Fluorescent Reagent
Sample Volume-0.5 mL
Shipping Conditions-Shipped in Dry Ice
Target Class-ADCs Antibodies Cell surface proteins GPCRs
Technology-TRF
Unit Size-1 vial