Revvity, 81HUIGG1NC, pHSense Human IgG1 Negative Control, 50µg

Overview
pHSense Eu Fab reagents are designed to monitor antibody or anti-drug conjugate (ADC), as well as receptor- and membrane protein-mediated internalization in real time. To confidently attribute observed effects to specific antigen binding, it is crucial to include proper controls. Test antibodies may engage in non-specific interactions - particularly when their Fc regions bind to Fc receptors on cells - which can contribute to background signal. This is where negative control antibodies become essential in pHSense assays. They help distinguish true signal from background noise by revealing non-specific binding effects, thereby enhancing the reliability of your internalization measurements.
The pHSense Human IgG1 Negative Control is a full-length recombinant IgG1 antibody, composed of both heavy and light chains. It serves as an ideal isotype control when evaluating fully human, humanized, or chimeric antibodies in conjunction with pHSense reagents. Application-Internalization
Brand-pHSense
Detection Modality-pH sensitive dye
Product Group-Fluorescent Reagent
Shipping Conditions-Shipped in Dry Ice
Target Class-ADCs Antibodies Cell surface proteins GPCRs
Target Species-Human
Technology-TRF
Unit Size-1 vial