Revvity, AD0400, LANCE Anti-6His Eu-Antibody, 1 mg

Overview
Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
LANCE (Lanthanide chelate excite) and LANCE® Ultra™ are TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (˜10 nm). This results in the emission of light at 665 nm (for ULight dye and APC). Application-Protein-Protein Interaction
Automation Compatible-Yes
Brand-LANCE Ultra
Conjugates-Anti-6X His
Detection Modality-LANCE
Fluorophore-Eu-W1024
Product Group-Antibody
Shipping Conditions-Shipped in Dry Ice
Target Class-Binding Assay
Technology-TR-FRET
Unit Size-1 mg